白蚁肠道纤维素降解菌的分离和鉴定

doi:10.11843/j.issn.0366-6964.2019.09.026

ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2019, Vol. 50 ›› Issue (9): 1951-1954.doi: 10.11843/j.issn.0366-6964.2019.09.026

• RESEARCH NOTES • Previous Articles

Isolation and Identification of Cellulose-degrading Bacteria in Termite Gut

MA Yujun¹, ZHANG Kai¹, WANG Lei¹, ZHANG Kang¹, ZHANG Hong², WANG Xuezhi¹, LI Jie¹, LIANG Zijing¹, ZHANG Jingyan^1*, LI Jianxi^1*

1. Chinese Veterinary Research Laboratory, Lanzhou Institute of Animal Husbandry and Veterinary Medicine, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China;
2. Gansu Animal Health Inspection Institute, Lanzhou 730000, China

Received:2019-01-22 Online:2019-09-23 Published:2019-09-23

Abstract

Abstract: In order to seek high cellulase-producing strains, the termites intestinal flora was studied. The bacteria in intestinal tract were isolated by the method of medium separation under aerobic and anaerobic conditions. Three cellulase activities in the screened strains were analyzed at 48 h, and the strains were identified by gene sequencing and biochemical analysis. Ten cellulase-producing bacteria strains were screened (CX1-10), and the ratio of the diameter of hydrolysis zone was the largest (2.78) in CX10. Filter Paper Enzyme (FPA) in CX10 had the highest activity, reaching 49.5 IU·mL^-1. Cellulose Endonuclease in CX9 had the highest activity, reaching 25.9 IU·mL^-1. Cellulose endoglucanases in CX8, CX9 and CX10 were 67.8, 70.4 and 95.0 IU·mL^-1, respectively. All of the screened strains were identified as Bacillus, Citrobacter and Serratia. In this study, 10 bacteria were successfully isolated from the intestines of lower termites, among which the CX10 synthesize cellulase activity was the highest, which was identified as Bacillus firms.

CLC Number:

X172

MA Yujun, ZHANG Kai, WANG Lei, ZHANG Kang, ZHANG Hong, WANG Xuezhi, LI Jie, LIANG Zijing, ZHANG Jingyan, LI Jianxi. Isolation and Identification of Cellulose-degrading Bacteria in Termite Gut[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(9): 1951-1954.

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Isolation and Identification of Cellulose-degrading Bacteria in Termite Gut

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